Basic Information

Strain Name
C57BL/6-Fcgrttm1(FCGRT)/Bcgen
Common Name
B-hFcRn mice
Background
C57BL/6
Catalog number
110001
Aliases 
FCGRT(Fc fragment of IgG receptor and transporter)

Description

The neonatal Fc receptor (The neonatal Fc receptor (FcRn), which is similar in structure to the MHC class I molecule and also associates with beta-2-macroglobulin, it is found in the placenta to help facilitate transport of mother’s IgG to the growing fetus in humans. FcRn extends the half-life of IgG and serum albumin by reducing lysosomal degradation in endothelial cells and bone-marrow derived cells. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine, TNF-α, and down-regulated by IFN-γ.

Targeting strategy

Gene targeting strategy for B-hFcRn mice. The human full-length FCGRT cDNA sequence was inserted in the Fcgrt exon2  of wild-type mice

Details

Pharmacokinetic experiments of hIgG based on B-hFcRn mice

There was no significant difference in clearance of hIgG between B-hFcRn mice and C57BL/6 mice. Serum hIgG is still detectable with ELISA assay 2 weeks after tail intravenous injection (IV) of human IgG (10 mg/kg ) in both B-hFcRn mice and C57BL/6 mice .

Pharmacokinetic experiments of Ipilimumab based on B-hFcRn mice

There was no significant difference in clearance of Ipilimumab (IgG1) between B-hFcRn mice and C57BL/6 mice at 10 mg/kg(n=5). Serum Ipilimumab is still detectable with ELISA assay 3 weeks after tail intravenous injection (IV) of Ipilimumab in both B-hFcRn mice and C57BL/6 mice .

Pharmacokinetic experiments of hIgG based on B-hFcRn mice

Publications using B-hFcRn mice

  1. Li L, Wu Z, Wu M, Qiu X, Wu Y, Kuang Z, Wang L, Sun T, Liu Y, Yi S, Jing H, Zhou S, Chen B, Wu D, Wu W, Liu J. IBI112, a selective anti-IL23p19 monoclonal antibody, displays high efficacy in IL-23-induced psoriasiform dermatitis. Int Immunopharmacol. 2020 Dec;89(Pt B):107008. doi: 10.1016/j.intimp.2020.107008. Epub 2020 Oct 15. PMID: 33069927.
  2. Wu F, Qiu Y, Xu Y. A single-valent long-acting human CD47 antagonist enhances antibody directed phagocytic activities. Cancer Immunol Immunother. 2020 Dec;69(12):2561-2569. doi: 10.1007/s00262-020-02640-6. Epub 2020 Jun 24. PMID: 32583154.
  3. Liu P, Pan Z, Gu C, Cao X, Liu X, Zhang J, Xiao Z, Wang X, Guo H, Ju D, Deng SJ. An Omalizumab Biobetter Antibody With Improved Stability and Efficacy for the Treatment of Allergic Diseases. Front Immunol. 2020 Nov 27;11:596908. doi: 10.3389/fimmu.2020.596908. PMID: 33329588; PMCID: PMC7728613.
  4. Qian Wang, Wangxi Hai, Sanyuan Shi, Jinliang Peng, Yuhong Xu, Oral uptake and persistence of the FnAb-8 protein characterized by in situ radio-labeling and PET/CT imaging, Asian Journal of Pharmaceutical Sciences, Volume 15, Issue 6, 2020, Pages 752-758, ISSN 1818-0876, https://doi.org/10.1016/j.ajps.2020.03.002.Oral uptake and persistence of the FnAb-8 protein characterized by in situ radio-labeling and PET/CT imaging