Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFGB mice.
The exons 1~8 of mouse Fgb gene that encode the full-length protein were replaced by human FGB exons 1~8 in B-hFGB mice.
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mRNA expression analysis
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Strain specific analysis of FGB mRNA expression in wild-type C57BL/6 mice and B-hFGB mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hFGB mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FGB primers. Mouse Fgb mRNA was detectable only in wild-type C57BL/6 mice. Human FGB mRNA was detectable only in homozygous B-hFGB mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of FGB protein expression in homozygous B-hFGB mice. Liver tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGB mice (H/H), and then analyzed by western blot with species-specific anti-FGB antibody. 40 μg total proteins were loaded for western blotting analysis. FGB was detected in liver.
Western blot analysis of FGB protein expression in homozygous B-hFGB mice. Plasma tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGB mice (H/H), and then analyzed by western blot with species-specific anti-FGB antibody. 10，20，and 30 μg total proteins were loaded for western blotting analysis. FGB was detected in plasma.
