Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFGFRL1 mice.
Exons 3-7 of mouse Fgfrl1, which encodes the extracellular domain, were replaced by human FGFRL1 exons 3-7 in B-hFGFRL1 mice.
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Protein expression analysis
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Western blot analysis of FGFRL1 protein expression in homozygous B-hFGFRL1 mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hFGFRL1 mice(v2) (H/H), and then analyzed by western blot with species-specific anti-FGFRL1 antibody. 40 μg total proteins were loaded for western blotting analysis. FGFRL1 was detected in lung.
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mRNA expression analysis
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Strain specific analysis of FGFRL1 mRNA expression in wild-type C57BL/6 mice and B-hFGFRL1 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hFGFRL1 mice(v2) (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FGFRL1 primers. Mouse Fgfrl1 mRNA was detectable only in wild-type C57BL/6 mice. Human FGFRL1 mRNA was detectable only in homozygous B-hFGFRL1 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Fgfrl1 was detectable in wild-type mice. Human FGFRL1 was detectable in homozygous B-hFGFRL1 mice.
Protein expression analysis:
Mouse FGFRL1 was detectable in lung of wild-type mice. Human FGFRL1 was detectable in lung of homozygous B-hFGFRL1 mice.
