Basic Information

Strain Name
C57BL/6N-F12tm1(F12)/Bcgen
Common Name
B-hFXII mice
Background
C57BL/6N
Catalog number
112750
Aliases 
HAE3, HAEX, HAF
NCBI Gene ID

mRNA expression analysis in humanized B-hFXII mice

Strain specific analysis of FXII mRNA expression in wild-type C57BL/6N mice and B-hFXII mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and heterozygous B-hFXII mice (H/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FXII primers. Mouse FXII mRNA were detectable in wild-type C57BL/6N and heterozygous B-hFXII mice. Human FXII mRNA was only detectable in heterozygous B-hFXII mice.

Protein expression analysis in humanized B-hFXII mice

Strain specific FXII expression analysis in heterozygous B-hFXII mice by ELISA. Serum and plasma were collected from wild-type mice C57BL/6N mice (+/+) and heterozygous B-hFXII mice (H/+) (n=2), and analyzed by ELISA with species-specific FXII ELISA kit. Human FXII was exclusively detectable in heterozygous mice but not in wild-type mice. Values are expressed as mean ± SEM. Significance was determined by unpaired t-test.  *P < 0.05, **P < 0.01, ***p < 0.0001.

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