Basic Information
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Protein expression analysis in serum
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Strain specific GCG expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hGCG mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGCG mice (H/H) (n=3, 7-week-old). Protein expression level of GLP1 and Glucagon were analyzed by ELISA. Human GLP1 and human Glucagon were exclusively detectable in homozygous B-hGCG mice.
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mRNA expression analysis in small intestine
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Species specific analysis of GCG gene expression in wild-type C57BL/6 mice and homozygous humanized B-hGCG mice by RT-PCR. Small intestine were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGCG mice (H/H). Mouse Gcg mRNA was detectable only in wild-type C57BL/6 mice. Human GCG mRNA was detectable only in homozygous B-hGCG mice, but not in wild-type C57BL/6 mice.
