Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hITGAV mice. The exons 1-30 of mouse Itgav gene were replaced by human ITGAV exons 1-30 in B-hITGAV mice.
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mRNA expression analysis
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Strain specific analysis of ITGAV gene expression in wild-type mice and B-hITGAV mice by RT-PCR. Mouse ITGAV mRNA was detectable in BM of wild-type mice (+/+). Human ITGAV mRNA was detectable only in homozygous B-hITGAV but not in wild-type mice.
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Protein expression analysis
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Strain-specific ITGAV expression analysis in homozygous B-hITGAV mice by flow cytometry. BM was collected from wild-type mice (+/+) and homozygous B-hITGAV mice(H/H) and analyzed by flow cytometry with species-specific anti-ITGAV antibody. Mouse ITGAV was detectable in wild-type mice. Human ITGAV was detected in homozygous B-hITGAV mice.
Strain-specific ITGAV expression analysis in homozygous B-hITGAV mice by flow cytometry. Blood samples were collected from wild-type mice (+/+) and homozygous B-hITGAV mice(H/H), and analyzed by flow cytometry with species-specific anti-ITGAV antibody. Mouse ITGAV was detectable in wild-type mice. Human ITGAV was detected in homozygous B-hITGAV mice.
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Summary
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mRNA expression：
Mouse ITGAV mRNA was detectable in BM of wild-type mice (+/+). Human ITGAV mRNA was detectable only in homozygous B-hITGAV but not in wild-type mice.
Protein expression:
Mouse ITGAV was detectable in wild-type mice. Human ITGAV was exclusively detectable in homozygous B-hITGAV mice but not in wild-type mice.
