Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hJAM3 mice. The exons 2-6 of mouse Jam3 gene that encode the extracellular domain were replaced by human JAM3 exons 2-6 in B-hJAM3 mice.
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mRNA expression analysis
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Species-specific JAM3 gene expression analysis in wild-type and humanized B-hJAM3 mice. Murine Jam3 mRNA was detected in lung tissue isolated from wild-type C57BL/6 (+/+) mice, while human JAM3 mRNA was detected in homozygous B-hJAM3 (H/H) mice.
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Protein expression analysis
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Species-specific JAM3 protein expression analysis in wild-type and humanized B-hJAM3 mice. Bone marrow-derived hematopoietic stem cells (HSCs) were isolated from wild-type C57BL/6 (+/+) and homozygous B-hJAM3 (H/H) mice and analyzed by flow cytometry using an anti-human JAM3 antibody. Human JAM3 protein was detected in B-hJAM3 mice.
Species-specific JAM3 protein expression analysis in wild-type and humanized B-hJAM3 mice. Lung-derived endothelial cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hJAM3 (H/H) mice and analyzed by flow cytometry using an anti-human JAM3 antibody. Human JAM3 protein was detected in B-hJAM3 mice.