Basic Information

Strain name
C57BL/6N-Olr1tm2(OLR1)Bcgen/Bcgen
Common name
B-hLOX1 mice
Background
C57BL/6N
Catalog number
111978
Aliases
LOX1, LOXIN, SLOX1, CLEC8A, SCARE1
NCBI gene ID

Targeting strategy

Gene targeting strategy for B-hLOX1 mice.

The exon 2-6 of mouse LOX-1 gene that encodes the extracellular domain and transmembrane region  was replaced by human LOX1 exon 2-6 in B-hLOX1 mice.

mRNA expression analysis

Strain specific analysis of LOX1 mRNA expression in wild-type C57BL/6 mice and B-hLOX1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LOX1 primers. Mouse Lox1 mRNA was detectable only in wild-type C57BL/6 mice. Human LOX1 mRNA was detectable only in homozygous B-hLOX1 mice but not in wild-type mice. And the PCR products were confirmed with Sanger sequencing.

Protein expression analysis

Strain specific LOX1 expression analysis in homozygous B-hLOX1 mice by flow cytometry. Bone marrow-derived dendritic cells(BMDC) were cultured from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H) stimulated with LPS  (1μg/mL , stimulation for 18 hour), and analyzed by flow cytometry with species-specific anti-LOX1 antibody. Mouse LOX1 was detectable on BMDC of wild-type mice. Human LOX1 was detectable on BMDC of homozygous B-hLOX1 mice.

Back to top
WordPress Double Opt-in by Forge12