Basic Information
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Targeting strategy
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Gene targeting strategy for B-hLSR mice.
The exons 1~5 of mouse Lsr gene that encode the extracellular domain were replaced by human LSR exons 1~5 in B-hLSR mice. -
Protein expression analysis
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Western blot analysis of LSR protein expression in homozygous B-hLSR mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hLSR mice (H/H), and then analyzed by western blot with anti-LSR antibody. 40 μg total proteins were loaded for western blotting analysis.
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mRNA expression analysis
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Strain specific analysis of LSR mRNA expression in wild-type C57BL/6 mice and B-hLSR mice by RT-PCR. Small intestine RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLSR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LSR primers. Mouse Lsr mRNA was detectable only in wild-type mice. Human LSR mRNA was detectable only in homozygous B-hLSR mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Lsr mRNA was detectable in small intestine of wild-type C57BL/6 mice. Human LSR mRNA was detectable in B-hLSR mice.Protein expression analysis:
Human LSR was detectable in uterus、small intestine、stomach and brain of homozygous B-hLSR mice.
