Basic Information

Strain Name
C57BL/6-Lsrtm1(LSR)/Bcgen
Common Name
B-hLSR mice
Background
C57BL/6
Catalog Number
112957
NCBI Gene ID
Related Genes
LSR: ILDR3; LISCH7

Targeting strategy

Gene targeting strategy for B-hLSR mice.
The exons 1~5 of mouse Lsr gene that encode the extracellular domain were replaced by human LSR exons 1~5 in B-hLSR mice.

Protein expression analysis

B-hLSR mice

Western blot analysis of LSR protein expression in homozygous B-hLSR mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hLSR mice (H/H), and then analyzed by western blot with anti-LSR antibody. 40 μg total proteins were loaded for western blotting analysis.

mRNA expression analysis

B-hLSR mice

Strain specific analysis of LSR mRNA expression in wild-type C57BL/6 mice and B-hLSR mice by RT-PCR. Small intestine RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLSR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LSR primers. Mouse Lsr mRNA was detectable only in wild-type mice. Human LSR mRNA was detectable only in homozygous B-hLSR mice but not in wild-type mice.

Summary

mRNA expression analysis:
Mouse Lsr mRNA was detectable in small intestine of wild-type C57BL/6 mice. Human LSR mRNA was detectable in B-hLSR mice.

Protein expression analysis:
Human LSR was detectable in uterus、small intestine、stomach and brain of homozygous B-hLSR mice.

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