Basic Information
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Protein expression analysis
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Western blot analysis of LUCA2 protein expression in homozygous B-hLUCA2 mice. Various tissue lysates were collected from C57BL/6N wildtype mice (+/+) and homozygous B-hLUCA2 mice (H/H), and then analyzed by western blot with anti-LUCA2 antibody. 40 μg total proteins were loaded for western blotting analysis. LUCA2 was detected in stomach, testis, lung, spleen, liver, and skeletal muscle in wild-type C57BL/6N mice and homozygous B-hLUCA2 mice.
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mRNA expression analysis
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Strain specific analysis of LUCA2 mRNA expression in wild-type C57BL/6 mice and B-hLUCA2 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hLUCA2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LUCA2 primers. Mouse Luca2 mRNA was detectable only in wild-type C57BL/6 mice. Human LUCA2 mRNA was detectable only in homozygous B-hLUCA2 mice but not in wild-type mice.
