Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPOSTN mice.
The exon 2-24 of mouse Postn gene that encodes the full-length protein was replaced by human POSTN exon 1-23 in B-hPOSTN mice. -
Protein expression analysis
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Strain specific POSTN expression analysis in homozygous B-hPOSTN mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPOSTN mice (H/H), serum was collected and analyzed by ELISA with species-specific POSTN ELISA kit. Mouse POSTN was detectable in wild-type mice (A). Human POSTN was exclusively detectable in homozygous B-hPOSTN mice (B).
Conclusion:
Protein expression: mouse POSTN was detectable in WT mice. Human POSTN was exclusively detectable in homozygous B-hPOSTN mice. -
Summary
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Protein expression analysis:
Human POSTN was detectable in homozygous B-hPOSTN mice, and mouse POSTN was detectable only in wild-type C57BL/6 mice.
