Basic Information
Strain Name
C57BL/6N-Pthtm1(PTH)Bcgen/Bcgen
Common Name
B-hPTH mice
Background
C57BL/6N
Catalog number
112633
Aliases
FIH11
NCBI Gene ID
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Protein expression analysis in serum
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Strain specific PTH expression analysis in homozygous B-hPTH mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPTH mice (H/H). Human PTH was exclusively detectable in homozygous B-hPTH mice.
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mRNA expression analysis
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Strain specific analysis of PTH mRNA expression in wild-type C57BL/6 mice and B-hPTH mice by RT-PCR. Thymus RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPTH mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human PTH primers. Mouse Pth mRNA was detectable only in wild-type C57BL/6 mice. Human PTH mRNA was detectable only in homozygous B-hPTH mice but not in wild-type mice.
