Basic Information

Strain name
C57BL/6-Ptk7tm1(PTK7)Bcgen/Bcgen
Common name
B-hPTK7 mice
Background
C57BL/6
Catalog number
112192
Aliases
CCK-4, CCK4
NCBI gene ID

Targeting strategy

Gene targeting strategy for B-hPTK7 mice.

Exon 2~14 of the mouse Ptk7 gene that encodes the full-length protein was replaced by human PTK7 exon 2~14 in B-hPTK7 mice.

mRNA expression analysis

Strain specific analysis of PTK7 mRNA expression in wild-type C57BL/6 mice and B-hPTK7 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPTK7 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human PTK7 primers. Mouse Ptk7 mRNA was detectable only in wild-type C57BL/6 mice. Human PTK7 mRNA was detectable only in homozygous B-hPTK7 mice but not in wild-type mice.

Protein expression analysis

Western blot analysis of PTK7 protein expression in homozygous B-hPTK7 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPTK7 mice (H/H), and then analyzed by western blot with anti-PTK7 antibody. 40 μg total proteins were loaded for western blotting analysis. PTK7 was detected in brain, lung and spleen from wild-type C57BL/6 mice and homozygous B-hPTK7 mice.

Back to top
WordPress Double Opt-in by Forge12