Basic Information
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Targeting strategy
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Gene targeting strategy for B-hSERPINF2 mice.
The exons 2-10 of mouse Serpinf2 gene that encodes the full-length protein were replaced by human SERPINF2 exons 2-10 in B-hSERPINF2 mice.
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Protein expression analysis
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Strain specific SERPINF2 expression analysis in homozygous B-hSERPINF2 mice by ELISA. Plasma was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hSERPINF2 mice (H/H) and analyzed by ELISA with species-specific SERPINF2 ELISA kit. Human SERPINF2 was exclusively detectable in homozygous B-hSERPINF2 mice but not in wild-type mice.
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mRNA expression analysis
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Strain specific analysis of SERPINF2 mRNA expression in wild-type C57BL/6 mice and B-hSERPINF2 mice by RT-PCR. Liver RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hSERPINF2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SERPINF2 primers. Mouse Serpinf2 mRNA was detectable in wild-type C57BL/6 mice. Human SERPINF2 mRNA was detectable only in homozygous B-hSERPINF2 mice but not in wild-type mice.
