Basic Information

Strain name
C57BL/6-Gt(ROSA)26tm1(SIGLEC8)/Bcgen
Common name
B-hSIGLEC8 mice
Background
C57BL/6
Catalog number
111149
Aliases
SAF2; SIGLEC-8; SIGLEC8L
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hSIGLEC8 mice. The BAC containing the whole sequence of human SIGLEC8 gene was inserted into Rosa26 locus in B-hSIGLEC8 mice.

Protein expression analysis

SIGLEC8 expression analysis in homozygous B-hSIGLEC8 mice by flow cytometry. Blood and Peritoneal lavage fluid were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSIGLEC8 mice (H/H), and analyzed by FACS with anti-SIGLEC8 antibody. Human SIGLEC8 was detectable in Eosinophils(88%, mSiglec-F+,mCD11c-) and mast cells (99.8%, mFcεRIα+,mCD117(c-kit)+,CD11b-) in homozygous B-hSIGLEC8 mice.

Eosinophils and mast cells in B-hSIGLEC8 mice bind to anti-human SIGLEC8 antibody

Analysis of eosinophils and mast cells of B-hSIGLEC8 mice by flow cytometry. Eosinophils and Peritoneal lavage-mast cells isolated from wild-type C57BL/6 mice (+/+) and B-hSIGLEC8 mice (H/H). Flow cytometry analysis was performed to assess human SIGLEC8 expression using the Benchmark antibody Lirentelimab (in house). Eosinophils were gated from mSiglec-F+,mCD11c- population and mast cells were gated from CD45+mFcεRIα+,mCD117(c-kit)+CD11b- population. Lirentelimab was exclusively binding to SIGLEC8 in homozygous B-hSIGLEC8 mice but not wild-type mice.

Summary

Protein expression analysis:

Human SIGLEC8 was detectable in Eosinophils(88%) and mast cells (99.8%) in homozygous B-hSIGLEC8 mice.