Basic Information
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Targeting strategy
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Gene targeting strategy for B-hSLAMF7 mice.
The exons 3~5 of mouse Slamf7 gene that encodes the full-length protein was replaced by human SLAMF7 exons 2~4 in B-hSLAMF7 mice. -
Protein expression analysis
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Strain specific SLAMF7 expression analysis in wild-type C57BL/6 and homozygous B-hSLAMF7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hSLAMF7 mice (H/H) and analyzed by flow cytometry with species-specific anti-SLAMF7 antibody. Mouse SLAMF7 was detectable only in T, B and NK cells in wild-type mice, and hSLAMF7 was exclusively detectable in T, B and NK cells in homozygous mice.
Strain specific SLAMF7 expression analysis in wild-type C57BL/6 and homozygous B-hSLAMF7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hSLAMF7 mice (H/H) and analyzed by flow cytometry with species-specific anti-SLAMF7 antibody. Mouse SLAMF7 was detectable only in monocytes and macrophages in wild-type mice, and hSLAMF7 was exclusively detectable in monocytes and macrophages in homozygous mice.
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Protein expression profiles of SLAMF7
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Strain specific SLAMF7 expression analysis in wild-type C57BL/6 and homozygous B-hSLAMF7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hSLAMF7 mice (H/H) and analyzed by flow cytometry with species-specific anti-SLAMF7 antibody. Mouse SLAMF7 was detectable in T, B, NK cells, monocytes and macrophages in wild-type mice, and hSLAMF7 was exclusively detectable in T, B, NK cells, monocytes and macrophages in homozygous mice.
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Summary
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Protein expression analysis:
Mouse SLAMF7 was detectable in T, B, NK cells, monocytes and macrophages in wild-type mice, and hSLAMF7 was exclusively detectable in T, B, NK cells, monocytes and macrophages in homozygous B-hSLAMF7 mice.
