Basic Information
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mRNA expression analysis
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Strain specific analysis of SLC16A3 mRNA expression in wild-type C57BL/6N mice and B-hSLC16A3 mice by RT-PCR. Skeletal muscle RNA samples were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hSLC16A3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SLC16A3 primers. Mouse Slc16a3 mRNA was detectable only in wild-type C57BL/6N mice. Human SLC16A3 mRNA was detectable only in homozygous B-hSLC16A3 mice.
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Protein expression analysis
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Western blot analysis of SLC16A3 protein expression in homozygous B-hSLC16A3 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hSLC16A3 mice (H/H), and then analyzed by simple-western blot with anti-SLC16A3 antibody. 3 μg total protein was loaded for western blotting analysis. SLC16A3 protein was detected in the brain, heart, liver, lung and skeletal muscle of wild-type C57BL/6N mice and homozygous B-hSLC16A3 mice, as the antibody recognizes both human and mouse SLC16A3.
