Basic Information
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mRNA expression analysis
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Strain specific analysis of SLC43A2 mRNA expression in wild-type C57BL/6J mice and B-hSLC43A2 mice by RT-PCR. Small intestine RNA samples were isolated from wild-type C57BL/6J mice (+/+) and homozygous B-hSLC43A2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse and human SLC43A2 primers. Mouse Slc43a2 mRNA was detectable only in wild-type C57BL/6J mice. Human SLC43A2 mRNA was detectable only in homozygous B-hLSLC43A2 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of SLC43A2 protein expression in homozygous B-hSLC43A2 mice. Various tissue lysates were collected from wild-type C57BL/6J (+/+) mice and homozygous B-hSLC43A2 mice (H/H), and then analyzed by western blot with anti-SLC43A2 antibody. 40μg total proteins were loaded for western blotting analysis. SLC43A2 was detected in kidney and large intestine of wild-type C57BL/6J mice and homozygous B-hSLC43A2 mice, as the antibodies used showed cross-recognition both for human and mouse SLC43A2. *The band sizes of intestine lane were ~70kDa, although bigger than that of kidney lane (predicted size), but was consistent with the published data.
