Basic Information
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Targeting strategy
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Gene targeting strategy for B-hSSTR2 mice.
The exon 2 of mouse Sstr2 gene that encode the extracellular and transmembrane domains was replaced by human SSTR2 exon 2 in B-hSSTR2 mice.
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mRNA expression analysis
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Strain specific analysis of SSTR2 mRNA expression in wild-type C57BL/6 mice and B-hSSTR2 mice by RT-PCR. Cerebellum RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hSSTR2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SSTR2 primers. Mouse Sstr2 mRNA was only detectable in wild-type C57BL/6 mice. Human SSTR2 mRNA was only detectable in homozygous B-hSSTR2 mice.
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Protein expression analysis
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Western blot analysis of SSTR2 protein expression in homozygous B-hSSTR2 mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hSSTR2 mice (H/H), and then analyzed by western blot with anti-SSTR2 antibody. 40 μg total proteins were loaded for western blotting analysis. SSTR2 was detected in cerebellum, pancreas, stomach, esophagus, uterus and colon.
