Basic Information

Strain name
Common name
B-hSSTR2 mice
Catalog number
NCBI gene ID
This product is used for the efficacy evaluation and safety evaluation of drugs for the treatment of neuroendocrine tumors.

Targeting strategy

Gene targeting strategy for B-hSSTR2 mice.

The exon 2 of mouse Sstr2 gene that encode the extracellular and transmembrane domains was replaced by human SSTR2 exon 2 in B-hSSTR2 mice.

mRNA expression analysis

Strain specific analysis of SSTR2 mRNA expression in wild-type C57BL/6 mice and B-hSSTR2 mice by RT-PCR. Cerebellum RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hSSTR2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SSTR2 primers. Mouse Sstr2 mRNA was only detectable in wild-type C57BL/6 mice. Human SSTR2 mRNA was only detectable in homozygous B-hSSTR2 mice.

Protein expression analysis

Western blot analysis of SSTR2 protein expression in homozygous B-hSSTR2 mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hSSTR2 mice (H/H), and then analyzed by western blot with anti-SSTR2 antibody. 40 μg total proteins were loaded for western blotting analysis. SSTR2 was detected in cerebellum, pancreas, stomach, esophagus, uterus and colon.

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