TIMP1(CLGI, EPA, EPO, HCI, TIMP, TIMP-1)
NCBI Gene ID
Gene targeting strategy for B-hTIMP1 mice.
The exons 2-6 of mouse Timp1 gene that encode the full-length protein, not including signal peptide were replaced by human TIMP1 exons 2-6 in B-hTIMP1 mice.
Protein expression analysis
Strain specific TIMP1 protein expression analysis in homozygous B-hTIMP1 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hTIMP1 mice(H/H), and analyzed by ELISA with species-specific TIMP1 ELISA kit. Mouse TIMP1 was detectable in wild-type mice. Human TIMP1 was exclusively detectable in homozygous B-hTIMP1 mice.
Protein expression analysis:
Human TIMP1 was exclusively detectable in homozygous B-hTIMP1 mice, and mouse TIMP1 was detectable only in wild-type mice.