Basic Information
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Targeting strategy
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Gene targeting strategy for B-hTLR4 mice.
Exons 1~3 of the mouse Tlr4 gene were replaced by human TLR4 exons 1~3 in B-hTLR4 mice. -
mRNA expression analysis
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Strain specific analysis of TLR4 mRNA expression in wild-type C57BL/6 mice and B-hTLR4 mice by RT-PCR. Spleen RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TLR4 primers. Mouse Tlr4 mRNA was detectable only in wild-type C57BL/6 mice, while human TLR4 mRNA was detectable only in homozygous B-hTLR4 mice.
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Protein expression analysis
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Strain specific TLR4 expression analysis in wild-type and homozygous B-hTLR4 mice by flow cytometry. Peritoneal cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTLR4 mice (H/H). Flow cytometry analysis of the peritoneal macrophages was detected with anti-TLR4 antibody. Mouse TLR4 was detected on peritoneal macrophages in wild-type mice. Human TLR4 was detected on peritoneal macrophages in homozygous B-hTLR4 mice.
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Summary
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mRNA expression analysis:
Mouse Tlr4 mRNA was detectable in splenocytes of wild-type C57BL/6 mice. Human TLR4 mRNA was detectable in B-hTLR4 mice.Protein expression analysis:
Human TLR4 was detectable on peritoneal macrophages in homozygous B-hTLR4 mice, and mouse TLR4 was detectable only in wild-type C57BL/6 mice.
