Basic Information
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Targeting strategy
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Gene targeting strategy for B-hUPA mice. The full coding region of mouse Upa was replaced by human UPA coding region sequences in B-hUPA mice.
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mRNA expression analysis
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Strain specific analysis of UPA gene expression in wild-type mice and B-hUPA mice by RT-PCR and western blot. (A) Mouse Upa mRNA was detectable in kidney of wild-type mice (+/+). Human UPA mRNA was detectable only in homozygous B-hUPA mice (H/H) but not in wild-type mice. (B) Liver and kidney tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hUPA mice (H/H), and analyzed by western blot with anti-UPA antibody. UPA was detectable in wild-type C57BL/6 mice (+/+) and homozygous B-hUPA mice (H/H), as the antibody is crossly reactive with both human and mouse UPA.
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Summary
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- mRNA expression analysis:
Human UPA mRNA was detectable only in homozygous B-hUPA mice but not in wild-type mice.
- Protein expression analysis:
UPA protein was detectable in wild-type C57BL/6 mice (+/+) and homozygous B-hUPA mice (H/H), as the antibody is crossly reactive with both human and mouse UPA.
