Basic Information
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Targeting strategy
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Gene targeting strategy for B-hZG16B mice.
The whole human ZG16B genome was inserted at Hipp11 (H11) locus in B-hZG16B mice.
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mRNA expression analysis
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Strain specific analysis of ZG16B mRNA expression in wild-type C57BL/6 mice and B-hZG16B mice by RT-PCR. Parotid gland RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hZG16B mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human ZG16B primers. Human ZG16B mRNA was detectable only in homozygous B-hZG16B mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of ZG16B protein expression in homozygous B-hZG16B mice. Various tissue lysates were collected from wild-type C57BL/6 mice and homozygous B-hZG16B mice (H/H), and then analyzed by western blot with anti-human ZG16B antibody. 40 μg total proteins were loaded for western blotting analysis. Human ZG16B was detected in prostate of homozygous B-hZG16B mice.
