Basic Information
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Targeting strategy
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Gene targeting strategy. The mouse uPA CDS driven by the Mup promoter, which was specific expression in the liver, was inserted into the mouse Hipp11 locus.
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Mouse uPA mRNA expression analysis by RT-qPCR
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Mouse uPA expression analysis in B-MUP-uPA mice by RT-qPCR. Liver and kidney tissues were isolated from wild-type C57BL/6 (+/+) and heterozygous B-MUP-uPA (H/+) mice (n=3, female, 9-week-old). Mouse uPA mRNA was expressed in the liver of heterozygous B-MUP-uPA mice and the level was higher than that in wild-type mice.
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ALT and AST concentrations in serum
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Serum ALT and AST analysis in B-MUP-uPA mice. Serum was collected from wild-type C57BL/6 (+/+) and heterozygous B-MUP-uPA (H/+) mice (n=10, male, 10-week-old). Serum ALT and AST concentrations were normal in B-MUP-uPA mice at 3 weeks of age, but elevated at 5 weeks, indicative of liver injury in B-MUP-uPA mice.
