Basic Information
Strain Name
C57BL/6JNifdc–Thy1tm1(Q108R)Bcgen/Bcgen
Stock Number
112848
Common Name
B-Thy1.1 mice
Background
C57BL/6JNifdc
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Targeting strategy
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Gene targeting strategy for B-Thy1.1 mice.
Introduce a glutamine to argnine acid mutation (Q108R) in mouse Thy1 gene, resulting that the encoding protein can be recognized by anti-Thy1.1 antibodies.
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Thy1.1 expression analysis in B-Thy1.1 mice
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Thy1.1 expression analysis in homozygous B-Thy1.1 mice by flow cytometry. Splenocytes, blood and thymocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-Thy1.1 mice (Mut/Mut), and then analyzed by flow cytometry with anti-Thy1.1 and anti-Thy1.2 antibodies. Thy1.2 was detectable in C57BL/6 mice. Thy1.1 were only detectable in B-Thy1.1 mice but not in wild-type mice.
