Basic Information

Strain Name
C57BL/6N-Abcg2tm1Bcgen/Bcgen
Common Name
B-Bcrp KO mice
Background
C57BL/6N
Catalog number
112776
Aliases
ABC15, ABCP, BCRP, Bcrp1, MXR, MXR1

mRNA expression analysis

Strain specific analysis of Abcg2 mRNA expression in wild-type C57BL/6 mice and B-Bcrp KO mice by RT-PCR. Brain and kidney RNA were isolated from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcg2 primers. Mouse Abcg2 mRNA was detectable in wild-type C57BL/6 mice (+/+) but not in B-Bcrp KO mice (-/-).

Protein expression analysis

Western blot analysis of BCRP protein expression in B-Bcrp KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and B-Bcrp KO mice (-/-), and then analyzed by western blot with anti-BCRP antibody. 40 μg total proteins were loaded for western blotting analysis. BCRP was detectable in kidney, liver, heart of wild-type C57BL/6 mice (+/+) but not B-Bcrp KO mice (-/-).

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