Reporter line that can be crossed with Cre-expressing strains. In presence of Cre, the floxed "stop" upstream of the TdTomato gene is deleted allowing expression of the red fluorescent TdTomato protein.
CAG promoter (a combination of artificially constructed promoter, which is composed of the early enhancer element of cytomegalovirus (CMV) and chicken beta-actin promoter)
A CAG-Loxp-Stop-Loxp-Tdtomato-PolyA sequence cassette was inserted into the ROSAS26 locus of the rat. This strain was maintained on a Sprague Dawley genetic background.
This is a reporter tool strain for Cre-dependent tdTomato fluorescent protein expression. In this strain, a loxp-stop-loxp cassette and tdTomato was placed under the CAG promoter.
B-CAG-iCre rats were bred to B-CAG-tdTomato cKI rats, tdTomato were detected
in the double-positive mice.