Basic Information

Strain Name
SD-Gt(ROSA)26Sortm1(CAG-LSL-tdtomato)/Bcgen
Common Name
B-CAG-tdTomato cKI rats
Background
SD
Catalog Number
112495
Application
Reporter line that can be crossed with Cre-expressing strains. In presence of Cre, the floxed "stop" upstream of the TdTomato gene is deleted allowing expression of the red fluorescent TdTomato protein.
Related Genes
CAG promoter (a combination of artificially constructed promoter, which is composed of the early enhancer element of cytomegalovirus (CMV) and chicken beta-actin promoter)
Appearance
White
Development

A CAG-Loxp-Stop-Loxp-Tdtomato-PolyA sequence cassette was inserted into the ROSAS26 locus of the rat. This strain was maintained on a Sprague Dawley genetic background.

Description

This is a reporter tool strain for Cre-dependent tdTomato fluorescent protein expression. In this strain, a loxp-stop-loxp cassette and tdTomato was placed under the CAG promoter.

Targeting strategy

B-CAG-tdTomato cKI rats

Phenotype Analysis

 

B-CAG-tdTomato cKI rats

B-CAG-iCre rats were bred to B-CAG-tdTomato cKI rats, tdTomato were detected in the double-positive mice.

Publication

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