B-CAG-Upar-tdTomato cKI mice

Basic Information

Strain Name
C57BL/6-Gt(ROSA)26Sortm1(CAG-LSL-mUPAR-IRES2-tdTomato)Bcgen/Bcgen
Common Name
B-CAG-Upar-tdTomato cKI mice
Background
C57BL/6
Catalog Number
111205
Application
B-CAG-Upar-tdTomato cKI mice model is an efficient tool to study the mouse UPAR function in different tissues after cross with the tissue specification Cre mice.
Related Genes
CD87, UPAR, URKR, U-PAR, PLAUR, ROSA26, tdTomato
Appearance
Black
Development

A CAG-LSL-mUPAR-IRES2-tdTomato-WPRE-pA cassette was placed between the exon 1 and exon 2 using C57BL/6 embryonic stem cells. This strain was maintained on a C57BL/6 genetic background.

Description

ROSAS26 gene produces a long non-coding RNA (lncRNA) that is under the control of a constitutive promoter. This locus is a widely used site for the integration of transgenes and reporter constructs

Targeting strategy

B-CAG-Upar-tdTomato cKI mice

Gene targeting strategy. The CAG-LSL-mUPAR-IRES2-tdTomato-WPRE-pA cassette that containing the full-length coding sequence of mouse Upar gene as well as tdTomato coding sequence were inserted into mouse Rosa26 gene locus site. The targeted mice crossed with Cre-expressing mice to implement tissue-specific expression.

Breeding & Genotyping

Case study: Cross with CMV-Cre mice, which can deletion of loxP-flanked genes occurs in all tissues, including germ cells.
B-CAG-Upar-tdTomato cKI mice

Protein expression analysis

B-CAG-Upar-tdTomato cKI mice

Mouse UPAR expression analysis in B-CAG-Upar-tdTomato cKI mice by flow cytometry. The B-CAG-Upar-tdTomato cKI mice were cross breeding with CMV-Cre mice, which is useful for deletion of loxP-flanked genes in all tissues. The splenocytes were collected from the offspring mice in UPAR (△/+) Cre (Tg/+) genotype, and analyzed by flow cytometry with anti-mouse UPAR antibody. As shown, the mUPAR was detectable in offspring mice. In addition, the tdTomato will co-expression as a reporter.

Publication

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