Basic Information

Strain Name
C57BL/6-Cd155tm1(EGFP)/Bcgen
Common Name
B-Cd155-EGFP KI mice
Background
C57BL/6N
Catalog number
111927
Aliases
CD155, HVED, NECL5, Necl-5, PVS, TAGE4
NCBI Gene ID
Application
Validation of the function of Cd155 in the development of tumor disease.

Description

Biocytogen designed a targeted mutation mouse named B-Cd155-EGFP KI mice which Cd155 gene was replaced with the EGFP gene.

Gene targeting strategy

Gene targeting strategy for B-Cd155-EGFP KI mice. The EGFP gene encoding green fluorescent protein was inserted after the 5 ‘UTR sequences of mouse Cd155 gene to replace it in B-Cd155-EGFP mice.

Protein expression analysis in B cell

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, bone marrow, lymph node and blood were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was not detectable in B cells of wild-type mice and homozygous B-Cd155-EGFP KI mice. EGFP was detectable in B cells of homozygous B-Cd155-EGFP KI mice.

Protein expression analysis in T cell

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, lymph node, thymic tissue and blood samples were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was detectable in T cells of wild-type mice, but not detectable in T cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in T cells of homozygous B-Cd155-EGFP KI mice.

Protein expression analysis in NK cell

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, thymus, bone marrow and lymph node samples were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was detectable in NK cells of wild-type mice, but not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice.

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Blood was collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was not detectable in NK cells of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in NK cells of homozygous B-Cd155-EGFP KI mice.

Protein expression analysis in macrophages

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood samples were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was detectable in macrophages of wild-type mice, but not detectable in macrophages of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in macrophages of homozygous B-Cd155-EGFP KI mice.

Protein expression analysis in monocytes

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Lavage solution, bone marrow, spleen and blood samples were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was detectable in monocytes of wild-type mice, but not detectable in monocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in monocytes of homozygous B-Cd155-EGFP KI mice.

Protein expression analysis in granulocytes

Species-specific mCD155 and EGFP protein expression analyses in homozygous B-Cd155-EGFP KI mice by flow cytometry. Spleen, blood and bone marrow were collected from wild-type mice (+/+) and homozygous B-Cd155-EGFP KI mice (-/-), and analyzed by flow cytometry with species-specific anti-CD155 and anti-EGFP antibodies. Mouse CD155 was detectable in granulocytes of wild-type mice, but not detectable in granulocytes of homozygous B-Cd155-EGFP KI mice. EGFP was detectable in granulocytes of homozygous B-Cd155-EGFP KI mice.

Summary

Protein expression analysis:

Mouse CD155 was detectable in T cells, NK cells, macrophages, monocytes and granulocytes of wild-type mice, but not homozygous B-Cd155-EGFP KI mice. EGFP was detectable in T cells, NK cells, macrophages, monocytes and granulocytes of homozygous B-Cd155-EGFP KI mice. 

Mouse CD155 was not detectable in B cells of wild-type mice and homozygous B-Cd155-EGFP KI mice, but EGFP was detectable in B cells of homozygous B-Cd155-EGFP KI mice. It is speculated that the expression level of CD155 in B cells of wild mice is too low to detect the expression. Or it was speculated that the targeting strategy affected the promoter of CD155, resulting in EGFP expression in B cells. 

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