Cell Gene Editing

Although gene edited animal models are very powerful tools for biomedical research, genetically modified cell lines have many advantages including convenience, fast timeline, and low cost.

In addition to our knockout cell lines, our gene editing services for knockin cell lines include free preliminary evaluation experiments, single cell colonization, genotyping and validation, tumor formation experiments, and FACS analysis.

Application Example #1:

A knockin cell line model was generated with EGFP inserted at the translation initiation site of ACTB (cytoskeleton filiform protein) in the U2OS cell line (human osteosarcoma). 

ACTB gene encodes β-actin, a cytoskeletal protein. The homologous arms of the targeting vector are about 1 kb, and the homologous recombination of the target vector was done by CRISPR/cas9 based EGE™ technology.

A similar gene editing strategy was used for LMNB1, which encodes the nucleoprotein Lamin-B1.

In U2OS cells, flow cytometry plots show that the efficiency of EGFP-ACTB gene knockin mediated by EGE™ (15.02%) is 8 times higher compared to CRISPR/Cas9-mediated knockin (1.91%). In the C6 cell line, the EGE™ system improves the knockin efficiency of EGFP-LMNB1 to 3.6% from 0.19%, which is a 19-fold increase.

Application Example #2:

Gene editing of hESCs and iPSCs

The highly efficient EGE™ system is ideal for editing the genomes of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) . 

Multiple iPSC lines are reprogrammed from human fibroblasts by non-integrated plasmids. Panel A shows phase contrast images. This iPSC knockin cell line expresses the pluripotency markers OCT4, SSEA-4 and TRA-1-60 as shown in Panel B and have a normal karyotype (Panel C) . Teratoma forms in immunodeficient mice (Panel D).

Human-Pluripotent-Stem-Cell

Humanized Tumor Cell Lines

Attention: Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Product BCG No. Organism Disease Culture Properties
B-CAG-hB7-H3 MC38 310704 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hB7-H4 MC38 310726 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hBCMA MC38 310720 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCD155 MC38 310697 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCD19 MC38 310706 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCD47 MC38 310695 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCD73 MC38 310728 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCEACAM1 MC38 310690 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hCLDN18.2 MC38 310715 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hEPCAM MC38 310708 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hHER2 MC38 310701 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hHVEM MC38 310694 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hPD-L1 (v2)/hCD47 MC38 320714 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hPD-L1 (v2)/hCEA MC38 320722 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hPD-L1 MC38 310685 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hPD-L1(v2) MC38 310699 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hPD-L2 MC38 310703 Mus musculus, mouse Colon Carcinoma Adherent
B-CAG-hROR1 MC38 310807 Mus musculus, mouse Colon Carcinoma Adherent
B-hCD47(v2) MC38 310696 Mus musculus, mouse Colon Carcinoma Adherent
B-hTNFR2 MC38 310723 Mus musculus, mouse Colon Carcinoma Adherent

Luciferase Reporter Tumor Cell Lines

Attention: Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Product Disease Organism Culture Type
B-CAG-luc Daudi Cell Line Burkitt’s lymphoma Human Suspension
B-CAG-luc Hep 3B (v2) Cell Line Hepatocellular carcinoma Human Adherent
B-CAG-luc U87-MG Cell Line Glioblastoma Human Adherent
B-Luciferase 4T1 Cell Line Breast carcinoma Murine Adherent
B-Luciferase B16-F10 Cell Line Melanoma Murine Adherent
B-Luciferase EL4 Cell Line Lymphoma Mouse Suspension
B-Luciferase Hep3B2.1-7 Cell Line Hepatocellular carcinoma Human Adherent
B-Luciferase Hepa 1-6 Cell Line Hepatoma Mouse Adherent
B-Luciferase K562 Cell Line Leukemia (CML) Human Suspension
B-Luciferase MIA PaCa-2 Cell Line Pancreatic carcinoma Human Adherent
B-Luciferase U87-MG Cell Line Glioblastoma Human Adherent
B-Luciferase-GFP HT-29 Cell Line Colorectal adenocarcinoma Human Adherent
B-Luciferase-GFP NCI-H1975 Cell Line Adenocarcinoma; non­small cell lung cancer Human Adherent
B-Luciferase-GFP Raji Cell Line Lymphoma, Burkitt’s Human Suspension

Humanized Tumor

Product BCG No. Organism Disease Culture Properties
B-hCEACAM1(v2) MC38 310691 Mus musculus, mouse Colon Carcinoma Adherent
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