Although gene edited animal models are very powerful tools for biomedical research, genetically modified cell lines have many advantages including convenience, fast timeline, and low cost.
In addition to our knockout cell lines, our gene editing services for knockin cell lines include free preliminary evaluation experiments, single cell colonization, genotyping and validation, tumor formation experiments, and FACS analysis.
Application Example #1:
A knockin cell line model was generated with EGFP inserted at the translation initiation site of ACTB (cytoskeleton filiform protein) in the U2OS cell line (human osteosarcoma).
ACTB gene encodes β-actin, a cytoskeletal protein. The homologous arms of the targeting vector are about 1 kb, and the homologous recombination of the target vector was done by CRISPR/cas9 based EGE™ technology.
A similar gene editing strategy was used for LMNB1, which encodes the nucleoprotein Lamin-B1.
In U2OS cells, flow cytometry plots show that the efficiency of EGFP-ACTB gene knockin mediated by EGE™ (15.02%) is 8 times higher compared to CRISPR/Cas9-mediated knockin (1.91%). In the C6 cell line, the EGE™ system improves the knockin efficiency of EGFP-LMNB1 to 3.6% from 0.19%, which is a 19-fold increase.
Application Example #2:
Gene editing of hESCs and iPSCs
The highly efficient EGE™ system is ideal for editing the genomes of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) .
Multiple iPSC lines are reprogrammed from human fibroblasts by non-integrated plasmids. Panel A shows phase contrast images. This iPSC knockin cell line expresses the pluripotency markers OCT4, SSEA-4 and TRA-1-60 as shown in Panel B and have a normal karyotype (Panel C) . Teratoma forms in immunodeficient mice (Panel D).
Humanized Tumor Cell Lines
Attention: Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
| Product | BCG No. | Organism | Disease | Culture Property |
|---|---|---|---|---|
| B-CAG-hB7-H3 MC38 | 310704 | Murine | Colon carcinoma | Adherent |
| B-CAG-hB7-H4 MC38 | 310726 | Murine | Colon carcinoma | Adherent |
| B-CAG-hBCMA MC38 | 310720 | Murine | Colon carcinoma | Adherent |
| B-CAG-hCD155 MC38 | 310697 | Murine | colorectal cancer | adherent |
| B-CAG-hCD19 MC38 | 310706 | Murine | Colon carcinoma | Adherent |
| B-CAG-hCD47 MC38 | 310695 | Murine | Colon carcinoma | Adherent |
| B-CAG-hCEACAM1 MC38 | 310690 | Murine | Colorectal cancer | Adherent |
| B-CAG-hCLDN18.2 MC38 | 310715 | Murine | colorectal cancer | adherent |
| B-CAG-hEPCAM MC38 | 310708 | Murine | Colon carcinoma | Adherent |
| B-CAG-hHER2 MC38 | 310701 | Murine | colorectal cancer | adherent |
| B-CAG-hHVEM MC38 | 310694 | Murine | colorectal cancer | adherent |
| B-CAG-hPD-L1 (v2)/hCD47 MC38 | 320714 | Murine | Colon Carcinoma | Adherent |
| B-CAG-hPD-L1 (v2)/hCEA MC38 | 320722 | Murine | Colon Carcinoma | Adherent |
| B-CAG-hPD-L1 MC38 | 310685 | Murine | colorectal cancer | adherent |
| B-CAG-hPD-L1 MC38 (v2) | 310699 | Murine | colorectal cancer | adherent |
| B-CAG-hPD-L2 MC38 | 310703 | Murine | colorectal cancer | adherent |
| B-hCD47 MC38(v2) | 310696 | Murine | colorectal cancer | adherent |
| B-hTNFR2 MC38 | 310723 | Murine | Colon Carcinoma | Adherent |
Luciferase Reporter Tumor Cell Lines
Attention: Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
| Product | Disease | Organism | Culture Type |
|---|---|---|---|
| B-CAG-luc Daudi Cell Line | Burkitt’s lymphoma | Human | Suspension |
| B-CAG-luc Hep 3B (v2) Cell Line | Hepatocellular carcinoma | Human | Adherent |
| B-CAG-luc U87-MG Cell Line | Glioblastoma | Human | Adherent |
| B-Luciferase 4T1 Cell Line | Breast carcinoma | Murine | Adherent |
| B-Luciferase B16-F10 Cell Line | Melanoma | Murine | Adherent |
| B-Luciferase EL4 Cell Line | Lymphoma | Mouse | Suspension |
| B-Luciferase Hep3B2.1-7 Cell Line | Hepatocellular carcinoma | Human | Adherent |
| B-Luciferase Hepa 1-6 Cell Line | Hepatoma | Mouse | Adherent |
| B-Luciferase K562 Cell Line | Leukemia (CML) | Human | Suspension |
| B-Luciferase MIA PaCa-2 Cell Line | Pancreatic carcinoma | Human | Adherent |
| B-Luciferase U87-MG Cell Line | Glioblastoma | Human | Adherent |
| B-Luciferase-GFP HT-29 Cell Line | Colorectal adenocarcinoma | Human | Adherent |
| B-Luciferase-GFP NCI-H1975 Cell Line | Adenocarcinoma; nonsmall cell lung cancer | Human | Adherent |
| B-Luciferase-GFP Raji Cell Line | Lymphoma, Burkitt’s | Human | Suspension |
Humanized Tumor
| Product | BCG No. | Organism | Disease | Culture Property |
|---|---|---|---|---|
| B-hCEACAM1 MC38(v2) | 310691 | Murine | Colon Carcinoma | Adherent |
