Basic Information

Strain name
C57BL/6-Cd36tm1(CD36)/Bcgen
Common name
B-hCD36 mice
Catalog number
111045
Background
C57BL/6
Related gene
CD36 (cluster of differentiation 36)
NCBI Gene ID

Basic information

CD36 targeting impairs intratumoral Treg cells and primes tumors to PD-1 blockade

Targeting strategy

Gene targeting strategy for B-hCD36 mice. The exons 4~14 of mouse Cd36 gene that encode the extracellular region were replaced by human CD36 exons 3~14 in B-hCD36 mice.

Details

Protein expression analysis in macrophages

Strain specific CD36 expression analysis in heterozygous B-hCD36 mice by flow cytometry. Bone marrow cells were collected from WT and heterozygous B-hCD36 (H/+) mice, and analyzed by flow cytometry with species-specific CD36 antibody. Mouse CD36 was detectable in WT mice and heterozygous B-hCD36. Human CD36 was exclusively detectable in heterozygous B-hCD36 but not WT mice.