Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCD36 mice. Exons 4~14 of mouse Cd36 gene that encode the extracellular region were replaced by human CD36 exons 3~14 in B-hCD36 mice.
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mRNA expression analysis
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Species-specific CD36 gene expression analysis in wild-type and humanized B-hCD36 mice by RT-PCR. Murine Cd36 mRNA was detected in lung tissue isolated from wild-type (+/+) mice, while human CD36 mRNA was exclusively detected in homozygous B-hCD36 (H/H) mice.
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Protein expression analysis in macrophages
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Species-specific CD36 protein expression analysis in humanized B-hCD36 mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD36 (H/+) mice and analyzed by flow cytometry using species-specific CD36 antibodies. Murine CD36 protein was detected in wild-type and heterozygous B-hCD36 mice, while human CD36 protein was exclusively detected in B-hCD36 mice.
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Complete blood count
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Complete blood count in wild-type and humanized B-hCD36 mice. Blood was isolated from wild-type C57BL/6 (+/+) and B-hCD36 mice (n=8, 6-8-week-old, female) and analyzed for complete blood count. Similar levels were observed between wild-type and B-hCD36 mice, indicating that replacing murine Cd36 with its human counterpart does not change blood cell composition. Values are expressed as mean ± SEM.
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Poster
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AACR 2023: Novel Humanized CD36 Mouse Model for Therapeutic Studies
