Basic Information

Strain name
C57BL/6-Cd36tm1(CD36)/Bcgen
Common name
B-hCD36 mice
Catalog number
111045
Background
C57BL/6
Related gene
CD36 (cluster of differentiation 36)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD36 mice. Exons 4~14 of mouse Cd36 gene that encode the extracellular region were replaced by human CD36 exons 3~14 in B-hCD36 mice.

mRNA expression analysis

Species-specific CD36 gene expression analysis in wild-type and humanized B-hCD36 mice by RT-PCR. Murine Cd36 mRNA was detected in lung tissue isolated from wild-type (+/+) mice, while human CD36 mRNA was exclusively detected in homozygous B-hCD36 (H/H) mice.

Protein expression analysis in macrophages

Species-specific CD36 protein expression analysis in humanized B-hCD36 mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD36 (H/+) mice and analyzed by flow cytometry using species-specific CD36 antibodies. Murine CD36 protein was detected in wild-type and heterozygous B-hCD36 mice, while human CD36 protein was exclusively detected in B-hCD36 mice.

Complete blood count

Complete blood count in wild-type and humanized B-hCD36 mice. Blood was isolated from wild-type C57BL/6 (+/+) and B-hCD36 mice (n=8, 6-8-week-old, female) and analyzed for complete blood count. Similar levels were observed between wild-type and B-hCD36 mice, indicating that replacing murine Cd36 with its human counterpart does not change blood cell composition. Values are expressed as mean ± SEM.

In vivo efficacy of an anti-human CD36 antibody

Antitumor activity of an anti-human CD36 antibody in humanized B-hCD36 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hCD36 mice (female, 8-week-old, n=6), when the tumor volume reached ~100mm3, mice were grouped and treated with different doses of an anti-human CD36 antibody. (A) The anti-human CD36 antibody (in house) inhibited MC38 tumor growth in B-hCD36 mice, (B) without negatively impacting body weight changes. Values are expressed as mean ± SEM.