Basic Information

Strain name
C57BL/6-Cd36tm1(CD36)/Bcgen
Common name
B-hCD36 mice
Catalog number
111045
Background
C57BL/6
Related gene
CD36 (cluster of differentiation 36)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD36 mice. Exons 4~14 of mouse Cd36 gene that encode the extracellular region were replaced by human CD36 exons 3~14 in B-hCD36 mice.

mRNA expression analysis

Species-specific CD36 gene expression analysis in wild-type and humanized B-hCD36 mice by RT-PCR. Murine Cd36 mRNA was detected in lung tissue isolated from wild-type (+/+) mice, while human CD36 mRNA was exclusively detected in homozygous B-hCD36 (H/H) mice.

Protein expression analysis in macrophages

Species-specific CD36 protein expression analysis in humanized B-hCD36 mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hCD36 (H/+) mice and analyzed by flow cytometry using species-specific CD36 antibodies. Murine CD36 protein was detected in wild-type and heterozygous B-hCD36 mice, while human CD36 protein was exclusively detected in B-hCD36 mice.

Complete blood count

Complete blood count in wild-type and humanized B-hCD36 mice. Blood was isolated from wild-type C57BL/6 (+/+) and B-hCD36 mice (n=8, 6-8-week-old, female) and analyzed for complete blood count. Similar levels were observed between wild-type and B-hCD36 mice, indicating that replacing murine Cd36 with its human counterpart does not change blood cell composition. Values are expressed as mean ± SEM.

In vivo efficacy of an anti-human CD36 antibody

Antitumor activity of an anti-human CD36 antibody in humanized B-hCD36 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hCD36 mice (female, 8-week-old, n=6), when the tumor volume reached ~100mm3, mice were grouped and treated with different doses of an anti-human CD36 antibody. (A) The anti-human CD36 antibody (in house) inhibited MC38 tumor growth in B-hCD36 mice, (B) without negatively impacting body weight changes. Values are expressed as mean ± SEM.

Anti-tumor activity of anti-mouse PD-1 antibody combined with anti-human CD36 antibody in B-hCD36 mice. (A) Anti-mPD-1 antibody combined with anti-hCD36 antibody inhibited MC38 tumor growth in B-hCD36 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hCD36 mice (female, 7 week-old, n=5). Mice were grouped when tumor volume reached approximately 60 mm3, at which time they were treated with antibodies in panel A. (B) Body weight changes during treatment. As shown in panel A, combination therapy was efficacious in controlling tumor growth in B-hCD36 mice, demonstrating that the B-hCD36 mice provide a powerful preclinical model for in vivo evaluation of anti-CD36 antibodies. The data is from our collaborator, all antibodies used in the experiment belong to the client. Values are expressed as mean ± SEM.