Gene targeting strategy for B-hCD36 mice. The exons 4~14 of mouse Cd36 gene that encode the extracellular region were replaced by human CD36 exons 3~14 in B-hCD36 mice.
Protein expression analysis in macrophages
Strain specific CD36 expression analysis in heterozygous B-hCD36 mice by flow cytometry. Bone marrow cells were collected from WT and heterozygous B-hCD36 (H/+) mice, and analyzed by flow cytometry with species-specific CD36 antibody. Mouse CD36 was detectable in WT mice and heterozygous B-hCD36. Human CD36 was exclusively detectable in heterozygous B-hCD36 but not WT mice.