Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hCD3E/HLA-A2.1 mice.
Exons 2-6 of mouse Cd3e (encoding the extracellular domain) were replaced by human CD3E exons 2-7 in B-hCD3E/HLA-A2.1 mice.
Exons 1-3 of mouse B2M were replaced by the sequence encompassing the human B2M CDS and HLA-A*0201 gene that includes leader sequence, α1 and α2 domains ligated to a fragment of the murine H-2Db gene containing the α3, transmembrane and cytoplasmic domains.
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Protein expression analysis
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Strain specific HLA expression analysis in homozygous B-hCD3E/HLA-A2.1 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and B-hCD3E/HLA-A2.1 mice, and analyzed by flow cytometry. Mouse H-2Db was detectable in WT mice (+/+) and B-hCD3E/HLA-A2.1 mice (CD3EH/H; HLA-A2.1H/+) . Human HLA-A2.1 was exclusively detected in B-hCD3E/HLA-A2.1 mice (CD3EH/H; HLA-A2.1H/+) and B-hCD3E/HLA-A2.1 mice (CD3EH/H; HLA-A2.1H/H) but not in WT mice (+/+).
Strain specific CD3E expression analysis in homozygous B-hCD3E/HLA-A2.1 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and B-hCD3E/HLA-A2.1 mice, and analyzed by flow cytometry with species-specific anti-hCD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in B-hCD3E/HLA-A2.1 mice (CD3EH/H; HLA-A2.1H/+) and B-hCD3E/HLA-A2.1 mice (CD3EH/H; HLA-A2.1H/H) but not in WT mice (+/+).
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