Basic Information
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mRNA expression analysis
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Species-specific VEGFR2 gene expression analysis in wild-type and humanized B-hVEGFR2 mice by RT-PCR.
Murine Vegfr2 mRNA was detected in embryonic tissue isolated from wild-type (+/+) mice, while human VEGFR2 mRNA was exclusively detected in homozygous B-hVEGFR2 (H/H) mice. -
Protein expression analysis
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Species-specific VEGFR2 protein expression analysis in wild-type and humanized B-hVEGFR2 mice. Embryonic lung endothelial cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hVEGFR2 (H/H) mice and analyzed by flow cytometry using anti-VEGFR2 antibodies. Human VEGFR2 protein was exclusively detected in B-hVEGFR2 mice.
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In vivo efficacy of an anti-human VEGFR2 antibody
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Antitumor activity of an anti-human VEGFR2 antibody in humanized B-hVEGFR2 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hVEGFR2 mice (female, 8-week-old, n=6), when the tumor volume reached ~100 mm3, mice were treated with an anti-human VEGFR2 antibody (Ramucirumab, in house). (A) Ramucirumab treatment inhibited MC38 tumor growth in B-hVEGFR2 mice, (B) without negatively impacting body weight changes. Values are expressed as mean ± SEM.
