mRNA expression analysis
Strain specific analysis of VEGFR2 gene expression in wild type and B-hVEGFR2 mice by RT-PCR.
Mouse Vegfr2 mRNA was detectable only in embryo of wild type (+/+) mice. Human VEGFR2 mRNA was detectable only in homozygous B-hVEGFR2 (H/H) mice but not in wild type mice.
Protein expression analysis
Strain specific VEGFR2 expression analysis in homozygous B-hVEGFR2 mice by flow cytometry.
Embryonic lung endothelial cells were collected from wild type (+/+) and homozygous B-hVEGFR2 (H/H) mice and analyzed by flow cytometry with anti-VEGFR2 antibody. Anti-Mouse VEGFR2 can cross-react with human/mouse VEGFR2 protein. Combined with mRNA expression detection in wild-type and homozygous B-hVEGFR2 mice This could prove that mouse VEGFR2 was detectable in wild type mice . Human VEGFR2 was exclusively detectable in homozygous B-hVEGFR2 but not in wild type mice.