Basic Information
Strain Name
C57BL/6-Apoetm1Bcgen/Bcgen
Stock Number
110168
Common Name
B-Apoe KO mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Apoe (apolipoprotein E); Apo-E; AI255918
Background
C57BL/6
Appearance
Black
Genotypes
ApoeKO (mut/mut);
Development
The ApoeKO mice were developed by Beijing Biocytogen Co., Ltd., the targeted mouse Apoe exons 3 was replaced with pGK-Neo cassette resulting in inactivation of Apoe protein. This strain was on a C57BL/6 genetic background.
NCBI Gene ID
-
Targeting Strategy
-
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Details
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Genotyping Information
Primer Sequence Tm （℃)
Product size (bp) ApoE-Primer-1 TGAGGCGGTTCTGAGACCTC 59 WT: 194 ApoE-Primer-2 TCAGAGTTCTAGAGGGAATTGGC 57 ApoE-Primer-1 TGAGGCGGTTCTGAGACCTC 59 WT: 826 Mut: 383
ApoE-Primer-3 GTCTCAAGAAGCCAAAAGCCAATC 58 Polymerase: 2XTSINGKE Master Mix（bluc）
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Publications
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- APOE2 promotes the development and progression of subretinal neovascularization in age-related macular degeneration via MAPKs signaling pathway
- Maintenance of AMPK activation by metformin is beneficial for suppressing the progress of diabetes-accelerated atherosclerosis via inhibition of vascular smooth muscle cells migration
- Betaine prevented high-fat diet-induced NAFLD by regulating the FGF10/AMPK signaling pathway in ApoE -/- mice
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References
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- Ishibashi S; Herz J; Maeda N; Goldstein JL; Brown MS. 1994. The two-receptor model of lipoprotein clearance: tests of the hypothesis in knockout mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins. Proc Natl Acad Sci U S A 91(10):4431-5. [PubMed: 8183926]
- Miura Y; Chiba T; Tomita I; Koizumi H; Miura S; Umegaki K; Hara Y; Ikeda M; Tomita T. 2001. Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. J Nutr 131(1):27-32. [PubMed: 11208934]
- Ekuni, D., et al., Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats. Lipids Health Dis, 2014. 13: p. 144. [PubMed: 25189624]
