Apolipoprotein E (ApoE) is primarily produced by the liver and macrophages in the peripheral tissues, or by astrocytes and microglia in the central nervous system. ApoE transports lipoproteins, fat-soluble vitamins and cholesterol into the lymph systems, and then into the blood, preventing the accumulation of cholesterol-rich particles in the plasma. The function of ApoE protein has been widely studied in cardiovascular disease, lipid metabolism, and Alzheimer’s diseases.
Primer Sequence Tm
Product size (bp) ApoE-Primer-1 TGAGGCGGTTCTGAGACCTC 59 WT: 194 ApoE-Primer-2 TCAGAGTTCTAGAGGGAATTGGC 57 ApoE-Primer-1 TGAGGCGGTTCTGAGACCTC 59 WT: 826
ApoE-Primer-3 GTCTCAAGAAGCCAAAAGCCAATC 58
Polymerase: 2XTSINGKE Master Mix（bluc）
- Ishibashi S; Herz J; Maeda N; Goldstein JL; Brown MS. 1994. The two-receptor model of lipoprotein clearance: tests of the hypothesis in knockout mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins. Proc Natl Acad Sci U S A 91(10):4431-5. [PubMed: 8183926]
- Miura Y; Chiba T; Tomita I; Koizumi H; Miura S; Umegaki K; Hara Y; Ikeda M; Tomita T. 2001. Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice. J Nutr 131(1):27-32. [PubMed: 11208934]
- Ekuni, D., et al., Occlusal disharmony accelerates the initiation of atherosclerosis in apoE knockout rats. Lipids Health Dis, 2014. 13: p. 144. [PubMed: 25189624]