Basic Information
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Targeting Strategy
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Phenotypic Analysis
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Phenotypic analysis of B-luc-GFP Raji. (A) Fluorescence microscopy showed green fluorescent protein expression in B-luc GFP Raji cells. (B) Luciferase expression was detected using the Bright-GloTM luciferase Assay System (Promega, Cat E2610). Fluorescence intensity of B-luc-GFP Raji cells was significantly higher compared to the control group.
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In Vivo Imaging Analysis
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Tumor growth and in vivo imaging of B-luc-GFP Raji cells. B-luc-GFP Raji cells (1×106) were injected by tail vein into B-NDG mice. (A) Imaging was performed on days 0, 4, 6, 8, 11, 15 and 18 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc-GFP Raji cells can be used for in vivo efficacy evaluation.
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Efficacy of Anti-Human CD47 Antibodies in Immunodeficient B-NDG mice
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B-luc-GFP Raji cells were injected intraperitoneally into B-NDG mice and observed with a small animal imager. Animals were enrolled into either the control group or the treatment group (n=6) when the tumor fluorescence intensity reached 1.5E6. (A) Various anti-human CD47 antibodies inhibited tumor growth, as indicated by tumor fluorescence intensity ± SEM, (B) without negatively impacting mean body weight (± SEM) changes.
