Basic Information

Cell Line Name
Raji Tg(luciferase)/Bcgen
Common Name
B-luc-GFP Raji
Catalog Number
310670
Cell Type/Disease
Burkitt’s lymphoma
Culture Properties
Suspension
Resistance
Blasticidin S HCl (BSH),10 μg/mL
Medium
RPMI-1640+10%FBS+2μg/mL BSH+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Digestive solution
N/A
Cryopreservation Medium
95% (Med)+5% DMSO

Targeting Strategy

Phenotypic Analysis

Phenotypic analysis of B-luc-GFP Raji. (A) Fluorescence microscopy showed green fluorescent protein expression in B-luc GFP Raji cells. (B) Luciferase expression was detected using the Bright-GloTM luciferase Assay System (Promega, Cat E2610). Fluorescence intensity of B-luc-GFP Raji cells was significantly higher compared to the control group.

In Vivo Imaging Analysis

Tumor growth and in vivo imaging of B-luc-GFP Raji cells. B-luc-GFP Raji cells (1×106) were injected by tail vein into B-NDG mice. (A) Imaging was performed on days 0, 4, 6, 8, 11, 15 and 18 for tumor detection and signal intensity analysis. (B) Body weight was measured twice a week (Mean ± SEM). Altogether these data indicate that B-luc-GFP Raji cells can be used for in vivo efficacy evaluation.

Efficacy of Anti-Human CD47 Antibodies in Immunodeficient B-NDG mice

B-luc-GFP Raji cells were injected intraperitoneally into B-NDG mice and observed with a small animal imager. Animals were enrolled into either the control group or the treatment group (n=6) when the tumor fluorescence intensity reached 1.5E6. (A) Various anti-human CD47 antibodies inhibited tumor growth, as indicated by tumor fluorescence intensity ± SEM, (B) without negatively impacting mean body weight (± SEM) changes.