Faster generation of antibody hits
Biocytogen’s antibody discovery platform harnesses the power of Berkeley Lights’ Beacon® Optofluidic system to generate antibody hits much faster than conventional hybridoma technology (from weeks to days). Unlike traditional discovery methods, single B cell antibody cloning has the distinct advantage of capturing positive hits early in the discovery process, which effectively eliminates the possibility that “good clones” are lost.
Beacon® single B cell antibody cloning workflow
From the animals exhibiting robust immune responses, we will isolate lymphoid tissue and enrich for B cells using magnetic bead technology. The Beacon® OptoFluidic system will detect antibodies secreted by single B cells that have high affinity for the labeled target antigen. The positive B cells are then lysed and sequenced for further analysis.
Single B cell antibody cloning technology
The Beacon® Optofluidic system uses light-sorting technology to profile the antigen-binding capacity of antibodies produced by a single B cell.
The B cell suspension is loaded into microfluidic chips, and each cell is individually housed in a chamber. The cells secreting antibodies that exhibit antigen-binding capacity are then individually exported for single B cell antibody cloning. This involves on-chip cell lysis and subsequent cDNA preparation. Once the cDNA is obtained and sequenced, the antibody sequences are expressed in cell lines for further validation studies.
Beacon® Optoselect chip technology.
Images created with BioRender.